Effect of Methanolic Extract of Hibiscus sabdariffa in Ethanol-Induced Hepatotoxicity

Egunlae Olanrewaju, Bond Anyaehie, Charles Ogbonna Ezeh, Kenechukwu Chibuike Onyekwelu,, Richard Chukwunonye Ezeh

Abstract


The liver is one of the most important organs in the body which aids in the maintenance of normal body functions. It is the center of alcohol metabolism and alcohol has been implicated in the elevation of liver enzymes and damage to the parenchyma cells of the liver. Plants had been used for medicinal purposes long before recorded history and many people worldwide rely on herbal medicines for some part of their primary health care. The objective of this study was to evaluate the activity of Hibiscus sabdariffa on the liver of rats following repeated administration of ethanol. Hepatotoxicity was induced on the rats using ethanol and the levels of serum enzymes such as serum glutamic pyruvic transaminase (SGPT), serum glutamic oxaloacetic transaminase (SGOT), total and conjugated bilirubin were estimated. The levels of hepatic enzymes ( SGOT, SGPT and bilirubin) in the serum were significantly increased (P < 0.05) in  rats treated with ethanol when compared with the control ( Pre phase). In the post phase, administration of different doses of ethanol extract significantly reduced the levels of increased hepatic serum enzymes when compared with the control. Methanolic extract of Hibiscus sabdariffa seems to have hepatoprotective effect on ethanol induced liver damage. However, similar study needs to be carried out on human to prove the authenticity of hepatoprotective effect of Hibiscus sabdariffa.


Keywords


Hibiscus sabdariffa, hepatoprotective, SGOT, SGPT and bilirubin

References


Navarro VJ, Senior JR (2006). Drug-related hepatotoxicity. N Engl J Med 2006; 354: 731-739.

Smart RG, Mann RE. (1992): Alcohol and the epidemiology of liver cirrhosis. Alcohol Health & Research World; 16(3):217–222.

National Center for Health Statistics. Health, United States, 1993. DHHS Pub. No. (PHS) 94-1232. Hyattsville, MD: the Center, 1994.

Rosalski SB, McIntyre N (1999): Biochemical investigations in the management of liver disease. In: Bircher J, Benhamou J-P, McIntyre N, Rizetto M, Rodes J, eds. Oxford handbook of clinical hepatology. 2nd ed. Oxford, England: Oxford University Press, 504.

Dake JA, Atchley AA (1984): Proximate analysis, In Christie, BR (ed). The handbook of plant science in agriculture, CRC Press, Inc., Boca Raton.

Tseng T, Kao T, Chu C, Chou F, Lin W, Wang C. (2000): Induction of apoptosis by hibiscus protocatechuic acid in human leukaemia cells via reduction of retinoblastoma (RB) phosphorylation and Bcl-2 expression. Biochemical Pharmacology. 60: 307-315.

Muhammad TB, Shakib AB (1995): Jus hibiscus: Bukan sekadar minuman biasa. Dewan Ekonomi 1995; 12-14.

Bako CV, Dawud G (2009): Drug induceed liver disease. Marcel Dekker Inc. New York U.S.A 2009; 773

Chang V, Huang MN. An overview of Hibiscus Sabdariffa. Natural Radiance product 2009; 8(1) 77-83

Trease GE, Evans WC (1989) Pharmacology 11th Edn., Bailliere Tindall Ltd., London 1989; 60-75

Lorke D (1983): A new approach to practical acute toxicity testing. Arch. Toxicol.; 54: 275-287.

Reitman S, Frankel S (1957): A colourimetric method for the determination of serum glutamate pyruvate transaminase and serum glutamate oxaloacetate transaminase. Am J Clin Pathol 28: 56-63.

Bessey, 0. A., Lowry, 0. H., and Brock, M. J.(1946): A method for the rapid determination of alkaline phosphatase with five cubic milliliters of serum. J. Biol. Chem. 164,321 (1946). Powel WN (1994): Determination of bilirubin in vertebrates. Annual Journal of Clinical Pathology 1994; 14:55 – 57.

Bako CV, Dawud G (2009): Drug induced liver disease. Marcel Dekker Inc. New York, USA 2009; 773

Agunwa CN (2004): Therapeutic basis of Clinical Pharmacy in Tropics 2004; 3rd Ed, 528-531.

Ndu OO (2007: Hepatoprotective Activity of Hibiscus sabdariffa in paracetamol induced Hepatotoxicity. Pharmacology Journal. 21: 45.

Zhou Q, Xie H, Zhang L, Stewart JK, Gu X, Ryan JJ (2006): Cis-Terpenones as an effective chemopreventive agent against aflotoxin B1 induced cytotoxicity and TCDD induced P4501 A/B activity in HepG2 cells. Chem. Res. Toxicol 2006; 19:1415-1419.

Packer L, Kagan VE (1993): Vitamin E: the antioxidant harvesting centre of membranes and lipoproteins. Marcel Dekker Inc., New York; 131-139.


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